Human LAMb3 (Laminin Beta 3) ELISA Kit | ELK3233

Human LAMb3 (Laminin Beta 3) ELISA Kit | ELK3233 | Gentaur UK, US & Europe Distribution

Alternative Names: LAM-B3; LAMNB1; Nicein subunit beta; Kalinin subunit beta; Epiligrin subunit bata; Laminin B1k chain; Laminin-5 subunit beta

Species: Human

Assay Type: Sandwich

Sensitivity: 0.26 ng/mL

Standard: 50 ng/mL

Detection Range: 0.79-50 ng/mL

Sample Type: Serum, plasma and other biological fluids.

Assay Lenght: 3.5h

Research Area: Metabolic pathway;Developmental science;Hepatology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Laminin Beta 3 (LAMb3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Laminin Beta 3 (LAMb3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Laminin Beta 3 (LAMb3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Laminin Beta 3 (LAMb3) in the samples is then determined by comparing the OD of the samples to the standard curve.