Human LAMC2 (Laminin Gamma 2) ELISA Kit | ELK3168

Human LAMC2 (Laminin Gamma 2) ELISA Kit | ELK3168 | Gentaur UK, US & Europe Distribution

Alternative Names: B2T; EBR2; EBR2A; LAMB2T; LAMNB2; Kalinin/nicein/epiligrin 100 kDa subunit; Cell-scattering factor 140 kDa subunit; Ladsin 140 kDa; Large adhesive scatter factor 140 kDa

Species: Human

Assay Type: Sandwich

Sensitivity: 6.7 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Developmental science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Laminin Gamma 2 (LAMC2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Laminin Gamma 2 (LAMC2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Laminin Gamma 2 (LAMC2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Laminin Gamma 2 (LAMC2) in the samples is then determined by comparing the OD of the samples to the standard curve.