Human Laminin subunit alpha-4 (LAMA4) ELISA Kit | KTE61861

Human Laminin subunit alpha-4 (LAMA4) ELISA Kit | KTE61861 | Gentaur UK, US & Europe Distribution

Application: This Human Laminin subunit alpha-4 (LAMA4) ELISA Kit employs a two-site sandwich ELISA to quantitate LAMA4 in samples. An antibody specific for LAMA4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLAMA4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LAMA4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LAMA4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Laminin subunit alpha-4 microplate
• Human Laminin subunit alpha-4 standard
• Human Laminin subunit alpha-4 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Laminin subunit alpha-4 (LAMA4) ELISA Kit has high sensitivity and excellent specificity for detection of Human LAMA4. No significant cross-reactivity or interference between Human LAMA4 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Laminin subunit alpha-4 isalpha chain isoform laminin, alpha 4. The domain structure of alpha 4 is similar to that of alpha 3, both of which resemble truncated versions of alpha 1 and alpha 2, in that approximately 1, 200 residues at the N-terminus (domains IV, V and VI) have been lost. Laminin, alpha 4 contains the C-terminal G domain which distinguishes all alpha chains from the beta and gamma chains. The RNA analysis from adult and fetal tissues revealed developmental regulation of expression, however, the exact function of laminin, alpha 4 is not known. Tissue-specific utilization of alternative polyA-signal has been described in literature. Also, alternative splicing involving the first intron in the 5' UTR, and laminin alpha 4 like isoforms have been noted, however, the full-length nature of these products is not known.

Alternative Names: LAMA4; RP1-142L7.4; DKFZp686D23145; LAMA3; LAMA4*-1; laminin alpha 4 chain

Search name: LAMA4; RP1-142L7.4; DKFZp686D23145; LAMA3; LAMA4*-1; laminin alpha 4 chain

Tag: LAMA4