Human Laminin subunit beta-3 (LAMB3) ELISA Kit | KTE61857

Human Laminin subunit beta-3 (LAMB3) ELISA Kit | KTE61857 | Gentaur UK, US & Europe Distribution

Application: This Human Laminin subunit beta-3 (LAMB3) ELISA Kit employs a two-site sandwich ELISA to quantitate LAMB3 in samples. An antibody specific for LAMB3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLAMB3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LAMB3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LAMB3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Laminin subunit beta-3 microplate
• Human Laminin subunit beta-3 standard
• Human Laminin subunit beta-3 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Laminin subunit beta-3 (LAMB3) ELISA Kit has high sensitivity and excellent specificity for detection of Human LAMB3. No significant cross-reactivity or interference between Human LAMB3 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: LAMB3 is a laminin that belongs to a family of basement membrane proteins. This protein is a beta subunit laminin, which together with an alpha and a gamma subunit, forms laminin-5. Mutations in this gene cause epidermolysis bullosa junctional Herlitz type, and generalized atrophic benign epidermolysis bullosa, diseases that are characterized by blistering of the skin. Multiple alternatively spliced transcript variants that encode the same protein have been found for this gene.LAMb3 is approximately 29 kb long and consists of 23 exons that vary from 64 to 379 basepairs, accounting for the full-length cDNA with an open reading frame of 3, 516 bp encoding 1, 172 amino acids. In comparison with the LAMB1 gene, LAMB3 is considerably more compact.

Alternative Names: LAMB3; RP1-272L16.3; BM600-125KDA; FLJ99565; LAM5; LAMNB1; kalinin B1 chain; kalinin-140kDa; laminin B1k chain; laminin S B3 chain; laminin; beta 3 (nicein (125kD) ; kalinin (140kD) ; BM600 (125kD) ) ; nicein-125kD

Search name: LAMB3; RP1-272L16.3; BM600-125KDA; FLJ99565; LAM5; LAMNB1; kalinin B1 chain; kalinin-140kDa; laminin B1k chain; laminin S B3 chain; laminin; beta 3 (nicein (125kD) ; kalinin (140kD) ; BM600 (125kD) ) ; nicein-125kD

Tag: LAMB3