Human Leucyl-cystinyl aminopeptidase (LNPEP) ELISA Kit | KTE61802

Human Leucyl-cystinyl aminopeptidase (LNPEP) ELISA Kit | KTE61802 | Gentaur UK, US & Europe Distribution

Application: This Human Leucyl-cystinyl aminopeptidase (LNPEP) ELISA Kit employs a two-site sandwich ELISA to quantitate LNPEP in samples. An antibody specific for LNPEP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLNPEP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LNPEP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LNPEP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Leucyl-cystinyl aminopeptidase microplate
• Human Leucyl-cystinyl aminopeptidase standard
• Human Leucyl-cystinyl aminopeptidase detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Leucyl-cystinyl aminopeptidase (LNPEP) ELISA Kit has high sensitivity and excellent specificity for detection of Human LNPEP. No significant cross-reactivity or interference between Human LNPEP and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: LNPEP encodes a zinc-dependent aminopeptidase that cleaves vasopressin, oxytocin, lys-bradykinin, met-enkephalin, dynorphin A and other peptide hormones. The protein can be secreted in maternal serum, reside in intracellular vesicles with the insulin-responsive glucose transporter GLUT4, or form a type II integral membrane glycoprotein. The protein catalyzes the final step in the conversion of angiotensinogen to angiotensin IV (AT4) and is also a receptor for AT4. Alternative splicing results in multiple transcript variants encoding different isoforms. The deduced 944-amino acid polypeptide contains the HEXXH consensus sequence of zinc metallopeptidases as well as an N-terminal hydrophobic region that resembles a transmembrane domain.

Alternative Names: LNPEP; CAP; IRAP; P-LAP; PLAP; AT (4) receptor; angiotensin IV receptor; insulin-regulated aminopeptidase; insulin-responsive aminopeptidase; otase; oxytocinase; placental leucine aminopeptidase; vasopressinase

Search name: LNPEP; CAP; IRAP; P-LAP; PLAP; AT (4) receptor; angiotensin IV receptor; insulin-regulated aminopeptidase; insulin-responsive aminopeptidase; otase; oxytocinase; placental leucine aminopeptidase; vasopressinase

Tag: LNPEP