Human Leukocyte cell derived chemotaxin 1 (LECT1) ELISA Kit | KTE61836

Human Leukocyte cell derived chemotaxin 1 (LECT1) ELISA Kit | KTE61836 | Gentaur UK, US & Europe Distribution

Application: This Human Leukocyte cell derived chemotaxin 1 (LECT1) ELISA Kit employs a two-site sandwich ELISA to quantitate LECT1 in samples. An antibody specific for LECT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLECT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LECT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LECT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Leukocyte cell derived chemotaxin 1 microplate
• Human Leukocyte cell derived chemotaxin 1 standard
• Human Leukocyte cell derived chemotaxin 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Leukocyte cell derived chemotaxin 1 (LECT1) ELISA Kit has high sensitivity and excellent specificity for detection of Human LECT1. No significant cross-reactivity or interference between Human LECT1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: LECT1 is a glycosylated transmembrane protein that is cleaved to form a mature, secreted protein. The N-terminus of the precursor protein shares characteristics with other surfactant proteins and is sometimes called chondrosurfactant protein although no biological activity has yet been defined for it. The C-terminus of the precursor protein contains a 25 kDa mature protein called leukocyte cell-derived chemotaxin-1 or chondromodulin-1. The mature protein promotes chondrocyte growth and inhibits angiogenesis. LECT1 expressed in the avascular zone of prehypertrophic cartilage and its expression decreases during chondrocyte hypertrophy and vascular invasion. The mature protein likely plays a role in endochondral bone development by permitting cartilaginous anlagen to be vascularized and replaced by bone.

Alternative Names: LECT1; RP11-93H24.1; BRICD3; CHM-I; CHM1; BRICHOS domain containing 3; chondromodulin I

Search name: LECT1; RP11-93H24.1; BRICD3; CHM-I; CHM1; BRICHOS domain containing 3; chondromodulin I

Tag: LECT1