Human Leukocyte immunoglobulin-like receptor subfamily A member 3 (LILRA3) ELISA Kit | KTE61814

Human Leukocyte immunoglobulin-like receptor subfamily A member 3 (LILRA3) ELISA Kit | KTE61814 | Gentaur UK, US & Europe Distribution

Application: This Human Leukocyte immunoglobulin-like receptor subfamily A member 3 (LILRA3) ELISA Kit employs a two-site sandwich ELISA to quantitate LILRA3 in samples. An antibody specific for LILRA3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLILRA3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LILRA3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LILRA3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Leukocyte immunoglobulin-like receptor subfamily A member 3 microplate
• Human Leukocyte immunoglobulin-like receptor subfamily A member 3 standard
• Human Leukocyte immunoglobulin-like receptor subfamily A member 3 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Leukocyte immunoglobulin-like receptor subfamily A member 3 (LILRA3) ELISA Kit has high sensitivity and excellent specificity for detection of Human LILRA3. No significant cross-reactivity or interference between Human LILRA3 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Leukocyte Ig-like receptors (LIRs) are a family of immunoreceptors expressed predominantly on monocytes and B cells and at lower levels on dendritic cells and natural killer (NK) cells. All LIRs in subfamily B have an inhibitory function . LIRs in subfamily A, with short cytoplasmic domains lacking an immunoreceptor tyrosine-based inhibitory motif (ITIM) and with transmembrane regions containing a charged arginine residue, may initiate stimulatory cascades . Acts as soluble receptor for class I MHC antigens. Binds both classical and non-classical HLA class I molecules but with reduced affinities compared to LILRB1 or LILRB2. Binds with high affinity to the surface of monocytes, leading to abolish LPS-induced TNF-alpha production by monocytes.

Alternative Names: LILRA3; CD85E; HM31; HM43; ILT6; LIR-4; LIR4; e3; leucocyte immunoglobulin-like receptor; leukocyte immunoglobulin-like receptor 4; leukocyte immunoglobulin-like receptor A3

Search name: LILRA3; CD85E; HM31; HM43; ILT6; LIR-4; LIR4; e3; leucocyte immunoglobulin-like receptor; leukocyte immunoglobulin-like receptor 4; leukocyte immunoglobulin-like receptor A3

Tag: LILRA3