Human Leukotriene A4 (LTA4)  ELISA Kit | AE63335HU

Human Leukotriene A4 (LTA4)  ELISA Kit | AE63335HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: LTA4

Alternative Name: N/A

Application: ELISA

Range: 123.5-10000 pg/mL

Sensitivity: 46.3 pg/mL

Intra-Assay: ≤4.6%

Inter-Assay: ≤7.8%

Recovery: 0, 95

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate LTA4 in samples. An antibody specific for LTA4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLTA4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LTA4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LTA4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Leukotriene A4 is a leukotriene.Leukotriene A4 hydrolase converts it to Leukotriene B4. (2s- (2 alpha, 3 beta (1e, 3e, 5z, 8z) ) ) -3- (1, 3, 5, 8-tetradecatetraenyl) oxiranebutanoic acid. An unstable allylic epoxide, formed from the immediate precursor 5-hpete via the stereospecific removal of a proton at c-10 and dehydration.  Leukotriene A4  (LTA4) is synthesized in mast cells, eosinophils, and neutrophils from arachidonic acid by 5-lipoxygenase (5-LO), which exhibits both lipoxygenase and LTA4 synthase activities.1, 2 LTA4 is rapidly metabolized by LTA4hydrolase or LTC4 synthase to LTB4 or LTC4, respectively.2 LTA4, from leukocytes, is known to undergo transcellular metabolism in platelets, erythrocytes, and endothelial cells.3 Further metabolism of LTA4 by 15-LO leads to lipoxin biosynthesis.2 LTA4 as a free acid is highly unstable. The methyl ester is stable and can be readily hydrolyzed to the free acid as needed.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .