Human LFA3 (Lymphocyte Function Associated Antigen 3) ELISA Kit | ELK2638

Human LFA3 (Lymphocyte Function Associated Antigen 3) ELISA Kit | ELK2638 | Gentaur UK, US & Europe Distribution

Alternative Names: CD58; Ag3; Surface glycoprotein LFA-3

Species: Human

Assay Type: Sandwich

Sensitivity: 5.8 pg/mL

Standard: 800 pg/mL

Detection Range: 12.5-800 pg/mL

Sample Type: Serum, plasma, cell lysatesand other biological fluids.

Assay Lenght: 3.5h

Research Area: CD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lymphocyte Function Associated Antigen 3 (LFA3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Lymphocyte Function Associated Antigen 3 (LFA3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lymphocyte Function Associated Antigen 3 (LFA3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lymphocyte Function Associated Antigen 3 (LFA3) in the samples is then determined by comparing the OD of the samples to the standard curve.