Human LH (Luteinizing Hormone) ELISA Kit | ELK5296

Human LH (Luteinizing Hormone) ELISA Kit | ELK5296 | Gentaur UK, US & Europe Distribution

Alternative Names: ICSH; Lutropin; Interstitial Cell Stimulating Hormone

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 0.45 mIU/mL

Standard: 85 mIU/mL

Detection Range: 1.33-85 mIU/mL

Sample Type: Serum, plasma and other biological fluids

Assay Lenght: 2h

Research Area: Endocrinology;Reproductive science;Hormone metabolism

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Luteinizing Hormone (LH) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Luteinizing Hormone (LH) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Luteinizing Hormone (LH) in the samples is then determined by comparing the OD of the samples to the standard curve.