Human LHb (Luteinizing Hormone Beta Polypeptide) ELISA Kit | ELK5381

Human LHb (Luteinizing Hormone Beta Polypeptide) ELISA Kit | ELK5381 | Gentaur UK, US & Europe Distribution

Alternative Names: LH-B; CGB4; LSH-B; hLHB; Lutropin Subunit Beta; Interstitial Cell Stimulating Hormone, Beta Chain

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 89.2 pg/mL

Standard: 20000 pg/mL

Detection Range: 312.5-20000 pg/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 2h

Research Area: Reproductive science

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Luteinizing Hormone Beta Polypeptide (LHb) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Luteinizing Hormone Beta Polypeptide (LHb) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Luteinizing Hormone Beta Polypeptide (LHb) in the samples is then determined by comparing the OD of the samples to the standard curve.