Human LIN28A (Lin-28 Homolog A) ELISA Kit | ELK7642

Human LIN28A (Lin-28 Homolog A) ELISA Kit | ELK7642 | Gentaur UK, US & Europe Distribution

Alternative Names: ZCCHC1; CSDD1; LIN28; Zinc finger CCHC domain-containing protein 1

Species: Human

Assay Type: Sandwich

Sensitivity: 21.9 pg/mL

Standard: 4000 pg/mL

Detection Range: 62.5-4000 pg/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lin-28 Homolog A (LIN28A) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Lin-28 Homolog A (LIN28A) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lin-28 Homolog A (LIN28A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lin-28 Homolog A (LIN28A) in the samples is then determined by comparing the OD of the samples to the standard curve.