Human Linker for activation of T cell (LAT) ELISA Kit | KTE61851

Human Linker for activation of T cell (LAT) ELISA Kit | KTE61851 | Gentaur UK, US & Europe Distribution

Application: This Human Linker for activation of T cell (LAT) ELISA Kit employs a two-site sandwich ELISA to quantitate LAT in samples. An antibody specific for LAT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLAT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LAT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LAT bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Linker for activation of T cell microplate
• Human Linker for activation of T cell standard
• Human Linker for activation of T cell detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Linker for activation of T cell (LAT) ELISA Kit has high sensitivity and excellent specificity for detection of Human LAT. No significant cross-reactivity or interference between Human LAT and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Linker of Activated T cells also known as LAT is a protein which is encoded by the LAT gene. Alternative splicing results in multiple transcript variants encoding different isoforms. The protein encoded by this gene is phosphorylated by ZAP70/SYK protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts (also known as glycosphingolipid-enriched microdomains or GEMs) and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement.

Alternative Names: LAT; LAT1; pp36; 36 kDa phospho-tyrosine adaptor protein; linker for activation of T-cells family member 1

Search name: LAT; LAT1; pp36; 36 kDa phospho-tyrosine adaptor protein; linker for activation of T-cells family member 1

Tag: LAT