Human Lipolysis-stimulated lipoprotein receptor (LSR) ELISA Kit | KTE61775

Human Lipolysis-stimulated lipoprotein receptor (LSR) ELISA Kit | KTE61775 | Gentaur UK, US & Europe Distribution

Application: This Human Lipolysis-stimulated lipoprotein receptor (LSR) ELISA Kit employs a two-site sandwich ELISA to quantitate LSR in samples. An antibody specific for LSR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLSR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LSR is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LSR bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Lipolysis-stimulated lipoprotein receptor microplate
• Human Lipolysis-stimulated lipoprotein receptor standard
• Human Lipolysis-stimulated lipoprotein receptor detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Lipolysis-stimulated lipoprotein receptor (LSR) ELISA Kit has high sensitivity and excellent specificity for detection of Human LSR. No significant cross-reactivity or interference between Human LSR and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: LSR is a multimeric protein complex in the liver that undergoes conformational changes upon binding of free fatty acids, thereby revealing a binding site (s) that recognizes both apoB and apoE. Complete inactivation of the LSR gene is embryonic lethal in mice.LSR cooperates with the LDL receptor in the final hepatic processing of apoB-containing lipoproteins and represents a novel therapeutic target for the treatment of hyperlipidemia associated with obesity and atherosclerosis. The lipolysis-stimulated receptor (LSR) is a lipoprotein receptor primarily expressed in the liver and activated by free fatty acids. Antibodies inhibiting LSR functions showed that the receptor is a heterotrimer or tetramer consisting of 68-kDa (alpha) and 56-kDa (beta) subunits associated through disulfide bridges.

Alternative Names: LSR; ILDR3; LISCH7; MGC10659; MGC48312; MGC48503; LISCH protein; immunoglobulin-like domain containing receptor 3; lipolysis-stimulated receptor; lipolysis-stimulated remnant; liver-specific bHLH-Zip transcr

Search name: LSR; ILDR3; LISCH7; MGC10659; MGC48312; MGC48503; LISCH protein; immunoglobulin-like domain containing receptor 3; lipolysis-stimulated receptor; lipolysis-stimulated remnant; liver-specific bHLH-Zip transcr

Tag: LSR