Human LNPEP (Leucyl/Cystinyl Aminopeptidase) ELISA Kit | ELK3824

Human LNPEP (Leucyl/Cystinyl Aminopeptidase) ELISA Kit | ELK3824 | Gentaur UK, US & Europe Distribution

Alternative Names: CAP; PLAP; OTASE; IRAP; Oxytocinase; Insulin-regulated membrane aminopeptidase; Insulin-responsive aminopeptidase; Placental leucine aminopeptidase

Species: Human

Assay Type: Sandwich

Sensitivity: 0.064 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leucyl/Cystinyl Aminopeptidase (LNPEP) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Leucyl/Cystinyl Aminopeptidase (LNPEP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Leucyl/Cystinyl Aminopeptidase (LNPEP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Leucyl/Cystinyl Aminopeptidase (LNPEP) in the samples is then determined by comparing the OD of the samples to the standard curve.