Human LPCAT4 (Lysophosphatidylcholine Acyltransferase 4) ELISA Kit | ELK4991

Human LPCAT4 (Lysophosphatidylcholine Acyltransferase 4) ELISA Kit | ELK4991 | Gentaur UK, US & Europe Distribution

Alternative Names: AYTL3; AGPAT7; LPAAT; LPEAT2; Lysophosphatidylethanolamine Acyltransferase 2; Acyltransferase Like 3; 1-Acylglycerol-3-Phosphate O-Acyltransferase 7; Plasmalogen synthase

Species: Human

Assay Type: Sandwich

Sensitivity: 0.34 ng/mL

Standard: 50 ng/mL

Detection Range: 0.79-50 ng/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lysophosphatidylcholine Acyltransferase 4 (LPCAT4) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Lysophosphatidylcholine Acyltransferase 4 (LPCAT4) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lysophosphatidylcholine Acyltransferase 4 (LPCAT4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Lysophosphatidylcholine Acyltransferase 4 (LPCAT4) in the samples is then determined by comparing the OD of the samples to the standard curve.