Human LRRC4B (Leucine Rich Repeat Containing Protein 4B) ELISA Kit | ELK7147

Human LRRC4B (Leucine Rich Repeat Containing Protein 4B) ELISA Kit | ELK7147 | Gentaur UK, US & Europe Distribution

Alternative Names: HSM; LRIG4; NGL-3; Netrin-G3 ligand; Leucine-Rich Repeats And Immunoglobulin-Like Domains 4

Species: Human

Assay Type: Sandwich

Sensitivity: 0.133 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Metabolic pathway

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leucine Rich Repeat Containing Protein 4B (LRRC4B) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Leucine Rich Repeat Containing Protein 4B (LRRC4B) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Leucine Rich Repeat Containing Protein 4B (LRRC4B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Leucine Rich Repeat Containing Protein 4B (LRRC4B) in the samples is then determined by comparing the OD of the samples to the standard curve.