Human Lung Tumor Blot VI | 1546

Human Lung Tumor Blot VI | 1546 | Gentaur UK, US & Europe Distribution

Tested Application: WB

Application: For Western Blot, the blot can be blocked with 1% BSA or 5% nonfat dry milk for 1 hour. Incubation takes 30 minutes to two hours with primary antibody, and 30 minutes to one hour with secondary antibody at room temperature.

Storage Condition: Store at 4°C. The blot can be reused after stripping.

User Note: Note: Lysate lane order may vary; please consult accompanying shipment paperwork.

Background: The human tissue blot is designed for proteomics research in screening for antibody and protein expression. It carries 1 normal human lung tissue, the cell line A549 (human lung carcinoma), and 7 different human lung tumor tissue lysates with 15 µg of total cellular protein in each lane. Proteins in a wide range of molecular mass are separated in a 4-20% gradient SDS-PAGE gel and transferred onto nitrocellulose membranes. The lung tumor tissues included in the blot are 1 small cell carcinoma, 1 non-small cell carcinoma, 1 differentiated adenocarcinoma, 1 squamous cell carcinoma, 1 pleomorphic carcinoma and 2 non-diagnosed carcinomas (from different donors) .

Shipping: N/A

Species: Human

Sample Loaded: Lanes: 1, protein markers; 2, A549; 3, normal lung tissue; 4, small cell carcinoma; 5, non-small cell carcinoma; 6, differentiated adenocarcinoma; 7, squamous cell carcinoma; 8, pleo-morphic carcinoma; 9, non-diagnosed carcinoma, 10, non-diagnosed carcinoma.

Quantity: 15 µg

Gel Percentage: Gradient 4-20%

Disclaimer: Products are intended for laboratory research purposes only and should be used by qualified personnel only. They are not intended for use in humans. ProSci is not liable for damages or injuries resulting from receipt and/or use of ProSci materials. Please refer to the Material Safety Data Sheet (MSDS) for safe storage, handling, and use procedures. Also, for further information on the biosafety classification of human etiologic agents, please consult the U.S. Department of Health and Human Services, Centers for Disease Control and Prevention's Office of Health and Safety (www.cdc.gov/od/ohs) .