Human Lutropin subunit beta (LHB) ELISA Kit | KTE61816

Human Lutropin subunit beta (LHB) ELISA Kit | KTE61816 | Gentaur UK, US & Europe Distribution

Application: This Human Lutropin subunit beta (LHB) ELISA Kit employs a two-site sandwich ELISA to quantitate LHB in samples. An antibody specific for LHB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLHB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LHB is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LHB bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Lutropin subunit beta microplate
• Human Lutropin subunit beta standard
• Human Lutropin subunit beta detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Lutropin subunit beta (LHB) ELISA Kit has high sensitivity and excellent specificity for detection of Human LHB. No significant cross-reactivity or interference between Human LHB and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Lutropin subunit beta is a member of the glycoprotein hormone beta chain family and encodes the beta subunit of luteinizing hormone (LH) . Glycoprotein hormones are heterodimers consisting of a common alpha subunit and a unique beta subunit which confers biological specificity. LH is expressed in the pituitary gland and promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids. The genes for the beta chains of chorionic gonadotropin and for luteinizing hormone are contiguous on chromosome 19q13.8. Mutations in this gene are associated with hypogonadism which is characterized by infertility and pseudohermaphroditism.

Alternative Names: LHB; CGB4; LSH-B; hLHB; interstitial cell stimulating hormone; beta chain; luteinizing hormone beta subunit; lutropin; beta chain

Search name: LHB; CGB4; LSH-B; hLHB; interstitial cell stimulating hormone; beta chain; luteinizing hormone beta subunit; lutropin; beta chain

Tag: LHB