Human Lysine-specific demethylase 4A (KDM4A) ELISA Kit | KTE61926

Human Lysine-specific demethylase 4A (KDM4A) ELISA Kit | KTE61926 | Gentaur UK, US & Europe Distribution

Application: This Human Lysine-specific demethylase 4A (KDM4A) ELISA Kit employs a two-site sandwich ELISA to quantitate KDM4A in samples. An antibody specific for KDM4A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKDM4A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KDM4A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KDM4A bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Lysine-specific demethylase 4A microplate
• Human Lysine-specific demethylase 4A standard
• Human Lysine-specific demethylase 4A detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Lysine-specific demethylase 4A (KDM4A) ELISA Kit has high sensitivity and excellent specificity for detection of Human KDM4A. No significant cross-reactivity or interference between Human KDM4A and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: JMJD2A encodes a deduced 1, 064-amino acid protein. Like JMJD2B and JMJD2C, JMJD2A contains a JmjN domain, a JmjC domain, a JD2H domain, 2 TUDOR domains, and a bipartite nuclear localization signal that overlaps the C-terminal part of the second TUDOR domain. JmjN is a small domain found in the Jumonji family of transcription factors. JmjC is a putative enzymatic domain. JD2H is a region containing 2 cys-his clusters with weak similarity to a PHD domain.This gene is a member of the Jumonji domain 2 (JMJD2) family and encodes a protein containing a JmjN domain, a JmjC domain, a JD2H domain, two TUDOR domains, and two PHD-type zinc fingers. This nuclear protein functions as a trimethylation-specific demethylase, converting specific trimethylated histone residues to the dimethylated form, and as a transcriptional repressor.

Alternative Names: KDM4A; JHDM3A; JMJD2; JMJD2A; KIAA0677; jumonji C domain-containing histone demethylase 3A; jumonji domain containing 2; jumonji domain containing 2A

Search name: KDM4A; JHDM3A; JMJD2; JMJD2A; KIAA0677; jumonji C domain-containing histone demethylase 3A; jumonji domain containing 2; jumonji domain containing 2A

Tag: KDM4A