Human Lysyl oxidase homolog 1 (LOXL1) ELISA Kit | KTE61800

Human Lysyl oxidase homolog 1 (LOXL1) ELISA Kit | KTE61800 | Gentaur UK, US & Europe Distribution

Application: This Human Lysyl oxidase homolog 1 (LOXL1) ELISA Kit employs a two-site sandwich ELISA to quantitate LOXL1 in samples. An antibody specific for LOXL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyLOXL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LOXL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LOXL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Lysyl oxidase homolog 1 microplate
• Human Lysyl oxidase homolog 1 standard
• Human Lysyl oxidase homolog 1 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Lysyl oxidase homolog 1 (LOXL1) ELISA Kit has high sensitivity and excellent specificity for detection of Human LOXL1. No significant cross-reactivity or interference between Human LOXL1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: LOXL1 encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family.Polymorphisms of the gene are associated with the pseudoexfoliation syndrome.

Alternative Names: LOXL1; LOL; LOXL; lysyl oxidase homolog 1

Search name: LOXL1; LOL; LOXL; lysyl oxidase homolog 1

Tag: LOXL1