Human Mannma binding protein/mannan binding lectin (MBP/MBL) ELISA Kit | KTE61702

Human Mannma binding protein/mannan binding lectin (MBP/MBL) ELISA Kit | KTE61702 | Gentaur UK, US & Europe Distribution

Application: This Human Mannma binding protein/mannan binding lectin (MBP/MBL) ELISA Kit employs a two-site sandwich ELISA to quantitate MBL2 in samples. An antibody specific for MBL2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMBL2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MBL2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MBL2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Mannma binding protein/mannan binding lectin microplate
• Human Mannma binding protein/mannan binding lectin standard
• Human Mannma binding protein/mannan binding lectin detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Mannma binding protein/mannan binding lectin (MBP/MBL) ELISA Kit has high sensitivity and excellent specificity for detection of Human MBL2. No significant cross-reactivity or interference between Human MBL2 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Mannosebinding protein (MBP), initiates the lectin branch of the innate immune response by binding to the surface of potentially pathogenic microorganisms and initiating complement fixation through an N-terminal collagen-like domain. MBL is a key component in immune response in that it can directly trigger neutralization of invading microorganisms by an Ab-independent mechanism.It binds to sugars on the surface of bacterial, fungal and parasitic cells through C-terminal, Ca2 -dependent carbohydrate-recognition domains. Mutations of human MBL are associated with immunodeficiency resulting from a reduction in the ability of the mutant MBL to initiate complement fixation.

Alternative Names: MBL2; COLEC1; HSMBPC; MBL; MBP; MBP1; MGC116832; MGC116833; mannan-binding lectin; mannose-binding lectin 2; soluble (opsonic defect) ; mannose-binding protein C; soluble mannose-binding lectin

Search name: MBL2; COLEC1; HSMBPC; MBL; MBP; MBP1; MGC116832; MGC116833; mannan-binding lectin; mannose-binding lectin 2; soluble (opsonic defect) ; mannose-binding protein C; soluble mannose-binding lectin

Tag: MBL2