Human MARCKS-related protein (MARCKSL1) ELISA Kit | KTE62234

Human MARCKS-related protein (MARCKSL1) ELISA Kit | KTE62234 | Gentaur UK, US & Europe Distribution

Application: This Human MARCKS-related protein (MARCKSL1) ELISA Kit employs a two-site sandwich ELISA to quantitate MARCKSL1 in samples. An antibody specific for MARCKSL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMARCKSL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MARCKSL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MARCKSL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human MARCKS-related protein microplate
• Human MARCKS-related protein standard
• Human MARCKS-related protein detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human MARCKS-related protein (MARCKSL1) ELISA Kit has high sensitivity and excellent specificity for detection of Human MARCKSL1. No significant cross-reactivity or interference between Human MARCKSL1 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: The myristoylated, alanine-rich protein MARCKS is a widely expressed, prominent substrate for protein kinase C, a key enzyme of intracellular signal transduction. The predicted 200-amino acid protein, which they called F52, shares 52% amino acid identity with bovine MARCKS. The similarity between the 2 proteins is found in the consensus myristoylation sequence near the N-terminus and in the 25-amino acid protein kinase C phosphorylation site domain. F52 has a similar amino acid composition to MARCKS, although its alanine content is not as high. It is distributed throughout the mouse brain in a pattern that is distinct from that of MARCKS.A transgene containing this 433-bp fragment from mouse linked to a reporter Mrp beta-galactosidase gene produced normal patterns of Mrp expression in the developing mouse embryo.

Alternative Names: MARCKSL1; F52; MACMARCKS; MLP; MLP1; MRP; MARCKS-like protein; MARCKS-related protein; macrophage myristoylated alanine-rich C kinase substrate

Search name: MARCKSL1; F52; MACMARCKS; MLP; MLP1; MRP; MARCKS-like protein; MARCKS-related protein; macrophage myristoylated alanine-rich C kinase substrate

Tag: MARCKSL1