Human MASP2 (Mannose Associated Serine Protease 2) ELISA Kit | ELK3024

Human MASP2 (Mannose Associated Serine Protease 2) ELISA Kit | ELK3024 | Gentaur UK, US & Europe Distribution

Alternative Names: Mannose-binding protein-associated serine protease 2; mannan-Binding Lectin Serine Peptidase 2; MBL-associated serine protease 2

Species: Human

Assay Type: Sandwich

Sensitivity: 13.9 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: serum, plasma and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase;Infection immunity;Immune molecule

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mannose Associated Serine Protease 2 (MASP2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mannose Associated Serine Protease 2 (MASP2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mannose Associated Serine Protease 2 (MASP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mannose Associated Serine Protease 2 (MASP2) in the samples is then determined by comparing the OD of the samples to the standard curve.