Human Mediator of RNA polymerase II transcription subunit 13 (MED13) ELISA Kit | KTE61669

Human Mediator of RNA polymerase II transcription subunit 13 (MED13) ELISA Kit | KTE61669 | Gentaur UK, US & Europe Distribution

Application: This Human Mediator of RNA polymerase II transcription subunit 13 (MED13) ELISA Kit employs a two-site sandwich ELISA to quantitate MED13 in samples. An antibody specific for MED13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMED13 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MED13 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MED13 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Mediator of RNA polymerase II transcription subunit 13 microplate
• Human Mediator of RNA polymerase II transcription subunit 13 standard
• Human Mediator of RNA polymerase II transcription subunit 13 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Mediator of RNA polymerase II transcription subunit 13 (MED13) ELISA Kit has high sensitivity and excellent specificity for detection of Human MED13. No significant cross-reactivity or interference between Human MED13 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: MED13 encodes a component of the mediator complex (also known as TRAP, SMCC, DRIP, or ARC), a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. The product of MED13 is proposed to form a sub-complex with MED12, cyclin C, and CDK8 that can negatively regulate transactivation by mediator. 

Alternative Names: MED13; ARC250; DRIP250; HSPC221; KIAA0593; THRAP1; TRAP240; activator-recruited cofactor 250 kDa component; mediator of RNA polymerase transcription; subunit 13 homolog; thyroid hormone receptor associate

Search name: MED13; ARC250; DRIP250; HSPC221; KIAA0593; THRAP1; TRAP240; activator-recruited cofactor 250 kDa component; mediator of RNA polymerase transcription; subunit 13 homolog; thyroid hormone receptor associate

Tag: MED13