Human melanin ELISA kit | ELK8367

Human melanin ELISA kit | ELK8367 | Gentaur UK, US & Europe Distribution

Alternative Names: melanin

Species: Human

Assay Type: Sandwich

Sensitivity: 12.5 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Cytokine;Infection immunity;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to melanin. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to melanin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain melanin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of melanin in the samples is then determined by comparing the OD of the samples to the standard curve.