Human MEP1a (Meprin A Alpha) ELISA Kit | ELK5367

Human MEP1a (Meprin A Alpha) ELISA Kit | ELK5367 | Gentaur UK, US & Europe Distribution

Alternative Names: PPHA; PABA Peptide Hydrolase; Endopeptidase-2; N-benzoyl-L-tyrosyl-P-amino-Benzoic Acid Hydrolase Subunit Alpha; PABA Peptide Hydrolase

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 17.4 pg/mL

Standard: 4000 pg/mL

Detection Range: 62.5-4000 pg/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 2h

Research Area: Metabolic pathway

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Meprin A Alpha (MEP1a) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Meprin A Alpha (MEP1a) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Meprin A Alpha (MEP1a) in the samples is then determined by comparing the OD of the samples to the standard curve.