Human MHCE (Major Histocompatibility Complex Class I E) ELISA Kit | ELK2168

Human MHCE (Major Histocompatibility Complex Class I E) ELISA Kit | ELK2168 | Gentaur UK, US & Europe Distribution

Alternative Names: HLAE; EA1.2; EA2.1; HLA-6.2; MHC-E; HLA-E; Leukocyte Antigen E; MHC class I antigen E; HLA class I histocompatibility antigen, alpha chain E

Species: Human

Assay Type: Sandwich

Sensitivity: 2.41 pg/mL

Standard: 400 pg/mL

Detection Range: 6.25-400 pg/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction;Tumor immunity;Infection immunity;Immune molecule;Developmental science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Major Histocompatibility Complex Class I E (MHCE) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Major Histocompatibility Complex Class I E (MHCE) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Major Histocompatibility Complex Class I E (MHCE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Major Histocompatibility Complex Class I E (MHCE) in the samples is then determined by comparing the OD of the samples to the standard curve.