Human MICA (MHC class I chain-related protein A) ELISA Kit | ELK8536

Human MICA (MHC class I chain-related protein A) ELISA Kit | ELK8536 | Gentaur UK, US & Europe Distribution

Alternative Names: major histocompatibility complex class I chain-related gene A; MHC class I chain-related protein B; MHC class I polypeptide related sequence A; MHC class I polypeptide related sequence B; MIC-A

Species: Human

Assay Type: Sandwich

Sensitivity: 0.39 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, urine, cerebrospinal fluid, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Immunology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to MHC class I chain-related protein A (MICA) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to MHC class I chain-related protein A (MICA) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain MHC class I chain-related protein A (MICA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of MHC class I chain-related protein A (MICA) in the samples is then determined by comparing the OD of the samples to the standard curve.