Human MIP3a (Macrophage Inflammatory Protein 3 Alpha) ELISA Kit | ELK1119

Human MIP3a (Macrophage Inflammatory Protein 3 Alpha) ELISA Kit | ELK1119 | Gentaur UK, US & Europe Distribution

Alternative Names: MIP3 Alpha; CCL20; CKb4; LARC; MIP3-A; SCYA20; ST38; Small Inducible Cytokine Subfamily A (Cys-Cys) Member 20; Beta-Chemokine Exodus-1; Liver And Activation-Regulated Chemokine

Species: Human

Assay Type: Sandwich

Sensitivity: 2.9 pg/mL

Standard: 500 pg/mL

Detection Range: 7.82-500 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Cytokine;Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Macrophage Inflammatory Protein 3 Alpha (MIP3a) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Macrophage Inflammatory Protein 3 Alpha (MIP3a) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Macrophage Inflammatory Protein 3 Alpha (MIP3a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Macrophage Inflammatory Protein 3 Alpha (MIP3a) in the samples is then determined by comparing the OD of the samples to the standard curve.