Human MIP5 (Macrophage Inflammatory Protein 5) ELISA Kit | ELK4574

Human MIP5 (Macrophage Inflammatory Protein 5) ELISA Kit | ELK4574 | Gentaur UK, US & Europe Distribution

Alternative Names: CCL15; SCYA15; HCC-2; NCC-3; SCYL3; Lkn-1; MIP1-D; MIP1d; MIP-1d; HMRP-2B; Leukotactin-1; Chemokine C-C-Motif Ligand 15; MIP-1 Delta; Small-inducible cytokine A15

Species: Human

Assay Type: Sandwich

Sensitivity: 6.2 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Cytokine;Tumor immunity;Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Macrophage Inflammatory Protein 5 (MIP5) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Macrophage Inflammatory Protein 5 (MIP5) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Macrophage Inflammatory Protein 5 (MIP5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Macrophage Inflammatory Protein 5 (MIP5) in the samples is then determined by comparing the OD of the samples to the standard curve.