Human Mixed lineage kinase domain-like (MLKL) ELISA Kit | KTE62368

Human Mixed lineage kinase domain-like (MLKL) ELISA Kit | KTE62368 | Gentaur UK, US & Europe Distribution

Application: This Human Mixed lineage kinase domain-like (MLKL) ELISA Kit employs a two-site sandwich ELISA to quantitate MLKL in samples. An antibody specific for MLKL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMLKL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MLKL is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MLKL bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Mixed lineage kinase domain-like microplate
• Human Mixed lineage kinase domain-like standard
• Human Mixed lineage kinase domain-like detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Mixed lineage kinase domain-like (MLKL) ELISA Kit has high sensitivity and excellent specificity for detection of Human MLKL. No significant cross-reactivity or interference between Human MLKL and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: MLKL, Belongs to the protein kinase superfamily. The protein kinase domain is predicted to be catalytically inactive. Protein kinases are critical modulators in the signal pathway of tumor cells and therefore a suitable object of drug development using inhibitors for such kinases. Sequence analysis of MLKL identified a kinase domain within the C-terminus of the protein. Further data support the idea that MLKL specifically can regulate more than one signaling pathway. However, the specific function of MLKL was not known yet. The apoptotic activity of MLKL was in fact higher compared to known tumor suppressors such as CDKN1A, PTEN, TP53, or MYC. These findings suggest MLKL as a new target for cancer diagnostic and/or therapy.

Alternative Names: MLKL; FLJ34389;

Search name: MLKL; FLJ34389;

Tag: MLKL