Human MMP17 (Matrix Metalloproteinase 17) ELISA Kit | ELK4789

Human MMP17 (Matrix Metalloproteinase 17) ELISA Kit | ELK4789 | Gentaur UK, US & Europe Distribution

Alternative Names: MT4-MMP; MMP4; Membrane-type matrix metalloproteinase 4; Membrane-type-4 matrix metalloproteinase

Species: Human

Assay Type: Sandwich

Sensitivity: 0.064 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase;Tumor immunity;Reproductive science;Developmental science;Rheumatology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Matrix Metalloproteinase 17 (MMP17) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Matrix Metalloproteinase 17 (MMP17) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Matrix Metalloproteinase 17 (MMP17), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Matrix Metalloproteinase 17 (MMP17) in the samples is then determined by comparing the OD of the samples to the standard curve.