Human Mothers against decapentaplegic homolog 1 (SMAD1) ELISA Kit | AE18186HU

Human Mothers against decapentaplegic homolog 1 (SMAD1) ELISA Kit | AE18186HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: SMAD1

Alternative Name: BSP1; JV4-1; JV41; MADH1; MADR1; MAD; mothers against decapentaplegic homolog 1|Mad-related protein 1|SMAD; mothers against DPP homolog 1|Sma- and Mad-related protein 1|TGF-beta signaling protein 1|

Application: ELISA

Range: 15.6-1000 pg/mL

Sensitivity: 6.6 pg/mL

Intra-Assay: ≤3.7%

Inter-Assay: ≤6.9%

Recovery: 1, 04

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate SMAD1 in samples. An antibody specific for SMAD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySMAD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SMAD1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SMAD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Glycyl-tRNA synthetase isone of the aminoacyl-tRNA synthetases that charge tRNAs with their cognate amino acids. The encoded enzyme is an (alpha) 2 dimer which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis.Defects in GARS are the cause of distal spinal muscular atrophy type V (DSMA-V) . DSMA-V is an autosomal dominant distal hereditary motor neuropathy (dHMN) with a phenotype similar to CMTD2. The main characteristic that distinguishes these disorders is the less severe distal sensory involvement in DSMA-V patients.Defects in GARS are the cause of Charcot-Marie-Tooth disease type 2D (CMT2D)

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .