Human MPIF2 (Myeloid Progenitor Inhibitory Factor 2) ELISA Kit | ELK1094

Human MPIF2 (Myeloid Progenitor Inhibitory Factor 2) ELISA Kit | ELK1094 | Gentaur UK, US & Europe Distribution

Alternative Names: CCL24; SCYA24; Ckb-6; Eotaxin-2; Chemokine C-C-Motif Ligand 24; Eosinophil chemotactic protein 2; Small Inducible Cytokine Subfamily A (Cys-Cys) Member 24

Species: Human

Assay Type: Sandwich

Sensitivity: 6.6 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Cytokine;Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myeloid Progenitor Inhibitory Factor 2 (MPIF2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Myeloid Progenitor Inhibitory Factor 2 (MPIF2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myeloid Progenitor Inhibitory Factor 2 (MPIF2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myeloid Progenitor Inhibitory Factor 2 (MPIF2) in the samples is then determined by comparing the OD of the samples to the standard curve.