Human MRC2 (Mannose Receptor C Type 2) ELISA Kit | ELK3857

Human MRC2 (Mannose Receptor C Type 2) ELISA Kit | ELK3857 | Gentaur UK, US & Europe Distribution

Alternative Names: CD280; ENDO180; CLEC13E; UPARAP; C-type lectin domain family 13 member E; Endocytic receptor 180; Macrophage mannose receptor 2; Urokinase receptor-associated protein

Species: Human

Assay Type: Sandwich

Sensitivity: 0.117 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids.

Assay Lenght: 3.5h

Research Area: Infection immunity;Developmental science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mannose Receptor C Type 2 (MRC2) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mannose Receptor C Type 2 (MRC2) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mannose Receptor C Type 2 (MRC2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mannose Receptor C Type 2 (MRC2) in the samples is then determined by comparing the OD of the samples to the standard curve.