Human MS4A1 (Membrane Spanning 4 Domains Subfamily A, Member 1) ELISA Kit | ELK4292

Human MS4A1 (Membrane Spanning 4 Domains Subfamily A, Member 1) ELISA Kit | ELK4292 | Gentaur UK, US & Europe Distribution

Alternative Names: CD20; MS4-A1; B1; Bp35; LEU16; MS4-A2; S7; B-lymphocyte antigen CD20; B-lymphocyte surface antigen B1

Species: Human

Assay Type: Sandwich

Sensitivity: 0.49 ng/mL

Standard: 80 ng/mL

Detection Range: 1.25-80 ng/mL

Sample Type: serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: CD & Adhesion molecule;Tumor immunity;Infection immunity;Immune molecule

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Membrane Spanning 4 Domains Subfamily A, Member 1 (MS4A1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Membrane Spanning 4 Domains Subfamily A, Member 1 (MS4A1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Membrane Spanning 4 Domains Subfamily A, Member 1 (MS4A1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Membrane Spanning 4 Domains Subfamily A, Member 1 (MS4A1) in the samples is then determined by comparing the OD of the samples to the standard curve.