Description
Human MSR1 (Macrophage Scavenger Receptor 1) ELISA Kit | ELK3334 | Gentaur UK, US & Europe Distribution
Alternative Names: CD204; MSR1; SCARA1; SR-A; PhSR1; PhSR2; Macrophage acetylated LDL receptor I and II; Scavenger receptor class A member 1
Species: Human
Assay Type: Sandwich
Sensitivity: 0.113 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids
Assay Lenght: 3.5h
Research Area: Signal transduction;Metabolic pathway;Infection immunity
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Macrophage Scavenger Receptor 1 (MSR1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Macrophage Scavenger Receptor 1 (MSR1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Macrophage Scavenger Receptor 1 (MSR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Macrophage Scavenger Receptor 1 (MSR1) in the samples is then determined by comparing the OD of the samples to the standard curve.