Human MUC1 (Mucin 1) ELISA Kit | ELK1201

Human MUC1 (Mucin 1) ELISA Kit | ELK1201 | Gentaur UK, US & Europe Distribution

Alternative Names: CD227; EMA; H23AG; KL-6; PEM; PEMT; PUM; CA15-3; Cancer antigen 15-3; Carcinoma-associated mucin; Episialin; Peanut-reactive urinary mucin; Polymorphic epithelial mucin

Species: Human

Assay Type: Sandwich

Sensitivity: 0.266 ng/mL

Standard: 40 ng/mL

Detection Range: 0.63-40 ng/mL

Sample Type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Tumor immunity;Infection immunity;Pulmonology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mucin 1 (MUC1) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mucin 1 (MUC1) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mucin 1 (MUC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mucin 1 (MUC1) in the samples is then determined by comparing the OD of the samples to the standard curve.