Human MUC5AC (Mucin 5 Subtype AC) ELISA Kit | ELK2098

Human MUC5AC (Mucin 5 Subtype AC) ELISA Kit | ELK2098 | Gentaur UK, US & Europe Distribution

Alternative Names: TBM; MUC5; LeB; Oligomeric Mucus/Gel-Forming; Mucin 5, Subtypes A And C, Tracheobronchial/Gastric; Gastric mucin; Lewis B blood group antigen; Major airway glycoprotein

Species: Human

Assay Type: Sandwich

Sensitivity: 30 pg/mL

Standard: 5000 pg/mL

Detection Range: 78.13-5000 pg/mL

Sample Type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Tumor immunity;Infection immunity;Endocrinology;Pulmonology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mucin 5 Subtype AC (MUC5AC) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mucin 5 Subtype AC (MUC5AC) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mucin 5 Subtype AC (MUC5AC), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mucin 5 Subtype AC (MUC5AC) in the samples is then determined by comparing the OD of the samples to the standard curve.