Human MUC5B (Mucin 5 Subtype B) ELISA Kit | ELK2122

Human MUC5B (Mucin 5 Subtype B) ELISA Kit | ELK2122 | Gentaur UK, US & Europe Distribution

Alternative Names: MUC5-B; MUC9; MG1; Oligomeric Mucus/Gel-Forming; Mucin 5, Subtype B, Tracheobronchial; Cervical mucin; High molecular weight salivary mucin MG1; Sublingual gland mucin

Species: Human

Assay Type: Sandwich

Sensitivity: 0.115 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Tumor immunity;Infection immunity;Pulmonology;Dermatology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mucin 5 Subtype B (MUC5B) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mucin 5 Subtype B (MUC5B) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mucin 5 Subtype B (MUC5B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mucin 5 Subtype B (MUC5B) in the samples is then determined by comparing the OD of the samples to the standard curve.