Human Myelin-associated glycoprotein (MAG) ELISA Kit | KTE61751

Human Myelin-associated glycoprotein (MAG) ELISA Kit | KTE61751 | Gentaur UK, US & Europe Distribution

Application: This Human Myelin-associated glycoprotein (MAG) ELISA Kit employs a two-site sandwich ELISA to quantitate MAG in samples. An antibody specific for MAG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMAG present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAG bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Myelin-associated glycoprotein microplate
• Human Myelin-associated glycoprotein standard
• Human Myelin-associated glycoprotein detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Myelin-associated glycoprotein (MAG) ELISA Kit has high sensitivity and excellent specificity for detection of Human MAG. No significant cross-reactivity or interference between Human MAG and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Myelin-associated glycoprotein (MAG) is a cell membrane glycoprotein that is a member of the SIGLEC family of proteins and is a functional ligand of the NOGO-66 receptor, NgR MAG is believed to be involved in myelination during nerve regeneration.Myelin-associated glycoprotein (MAG), a membrane protein of 100 kD, is thought to be involved in the process of myelination (Barton et al., 1987) . MAG is a sialic acid-binding SIGLEC protein and is a functional ligand for the NOGO receptor (NGR, or RTN4R) (Liu et al., 2002) .Spagnol et al. (1989) found that MAG cDNA clones isolated from a human brain cDNA library had an open reading frame of 1, 878 nucleotides encoding a peptide of 626 amino acids with a calculated molecular weight of 69.3 kD.

Alternative Names: MAG; GMA; S-MAG; SIGLEC-4A; SIGLEC4A; myelin-associated glycoprotein; sialic acid-binding immunoglobulin-like lectin 4A

Search name: MAG; GMA; S-MAG; SIGLEC-4A; SIGLEC4A; myelin-associated glycoprotein; sialic acid-binding immunoglobulin-like lectin 4A

Tag: MAG