Human Myotubularin-related protein 14 (MTMR14) ELISA Kit | KTE62366

Human Myotubularin-related protein 14 (MTMR14) ELISA Kit | KTE62366 | Gentaur UK, US & Europe Distribution

Application: This Human Myotubularin-related protein 14 (MTMR14) ELISA Kit employs a two-site sandwich ELISA to quantitate MTMR14 in samples. An antibody specific for MTMR14 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMTMR14 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MTMR14 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MTMR14 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Detection Method: Colorimetric

Conjugate: N/A

Sample Type: Cell culture supernatants#Serum#Plasma#Other biological fluids

Assay Type: Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Kit Component: • Human Myotubularin-related protein 14 microplate
• Human Myotubularin-related protein 14 standard
• Human Myotubularin-related protein 14 detect antibody
• Streptavidin-HRP
• Standard diluent
• Assay buffer
• HRP substrate
• Stop solution
• Wash buffer
• Plate covers

Features & Benefits: Human Myotubularin-related protein 14 (MTMR14) ELISA Kit has high sensitivity and excellent specificity for detection of Human MTMR14. No significant cross-reactivity or interference between Human MTMR14 and analogues was observed.

Calibration Range: Please inquire

Limit Of Detection: Please inquire

Usage Note: • Do not mix components from different kit lots or use reagents beyond the kit expiration date.
• Allow all reagents to warm to room temperature for at least 30 minutes before opening.
• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.
• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
• It is recommended that all samples and standards be assayed in duplicate or triplicate.

Storage Instruction: The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols.

Shipping: Gel pack with blue ice.

Precaution The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Background: Expression of Mtmr14 increased with myotubule formation and differentiation. MTMR14 is a phosphatidylinositol-3-phosphatase that dephosphorylates the same substrates as myotubularin, PtdIns (4, 5) P2 and PtdIns (3, 4, 5) P3. MTMR14encodes a myotubularin-related protein. The encoded protein is a phosphoinositide phosphatase that specifically dephosphorylates phosphatidylinositol 3, 5-biphosphate and phosphatidylinositol 3-phosphate. Mutations in this gene are correlated with autosomal dominant centronuclear myopathy. Alternate splicing results in multiple transcript variants. A pseudogene of this gene is found on chromosome 18. Expressed in various tissues, including heart, skeletal muscle, placenta, liver, lung, kidney and pancreas.

Alternative Names: MTMR14; C3orf29; FLJ22405; FLJ46453; FLJ90311; HJUMPY; hEDTP; HCV NS5A-transactivated protein 4 splice variant A-binding protein 1; NS5ATP4ABP1; egg-derived tyrosine phosphatase homolog; jumpy

Search name: MTMR14; C3orf29; FLJ22405; FLJ46453; FLJ90311; HJUMPY; hEDTP; HCV NS5A-transactivated protein 4 splice variant A-binding protein 1; NS5ATP4ABP1; egg-derived tyrosine phosphatase homolog; jumpy

Tag: MTMR14