Human NAD-dependent deacetylase sirtuin-1 (SIRT1/SIR2L1) ELISA Kit | AE63115HU

Human NAD-dependent deacetylase sirtuin-1 (SIRT1/SIR2L1) ELISA Kit | AE63115HU | Gentaur UK, US & Europe Distribution

Species Reactivity: Human (Homo sapiens)

Abbreviation: SIRT1

Alternative Name: RP11-57G10.3; SIR2L1; SIR2alpha|sir2-like 1|sirtuin 1|sirtuin type 1

Application: ELISA

Range: 0.78-50 ng/mL

Sensitivity: 0.28 ng/mL

Intra-Assay: ≤7.4%

Inter-Assay: ≤11.1%

Recovery: 1, 08

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate SIRT1 in samples. An antibody specific for SIRT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySIRT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SIRT1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SIRT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Sirtuin 1 is a member of the sirtuin family of proteins, homologs of the Sir2 gene in S. cerevisiae. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family.Sirtuin 1 is downregulated in cells that have high insulin resistance and inducing its expression increases insulin sensitivity, suggesting the molecule is associated with improving insulin sensitivity.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .