Human NAP3 (Neutrophil Activating Protein 3) ELISA Kit | ELK1024

Human NAP3 (Neutrophil Activating Protein 3) ELISA Kit | ELK1024 | Gentaur UK, US & Europe Distribution

Alternative Names: CXCL1; GRO1; GROa; GRO-A; MGSA-A; NAP3; SCYB1; Chemokine C-X-C-Motif Ligand 1; Melanoma Growth Stimulating Activity Alpha; Fibroblast Secretory Protein

Species: Human

Assay Type: Sandwich

Sensitivity: 6.1 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Lenght: 3.5h

Research Area: Cytokine;Tumor immunity;Infection immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neutrophil Activating Protein 3 (NAP3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Neutrophil Activating Protein 3 (NAP3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neutrophil Activating Protein 3 (NAP3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neutrophil Activating Protein 3 (NAP3) in the samples is then determined by comparing the OD of the samples to the standard curve.