Human NCCT (Na-Cl Cotransporter) ELISA Kit | ELK3971

Human NCCT (Na-Cl Cotransporter) ELISA Kit | ELK3971 | Gentaur UK, US & Europe Distribution

Alternative Names: SLC12A3; TSC; NCC; Solute Carrier Family 12 Member 3, Sodium/Chloride Transporters; Thiazide-sensitive sodium-chloride cotransporter; Na-Cl symporter

Species: Human

Assay Type: Sandwich

Sensitivity: 0.115 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Lenght: 3.5h

Research Area: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Na-Cl Cotransporter (NCCT) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Na-Cl Cotransporter (NCCT) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Na-Cl Cotransporter (NCCT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Na-Cl Cotransporter (NCCT) in the samples is then determined by comparing the OD of the samples to the standard curve.