Human NEDD4-L (E3 ubiquitin-protein ligase NEDD4-like) ELISA Kit | ELK9502

Human NEDD4-L (E3 ubiquitin-protein ligase NEDD4-like) ELISA Kit | ELK9502 | Gentaur UK, US & Europe Distribution

Alternative Names: NED4L；NEDD4 2

Species: Human

Assay Type: Sandwich

Sensitivity: 0.119 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: serum, plasma, tissue homogenates and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to E3 ubiquitin-protein ligase NEDD4-like (NEDD4-L) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to E3 ubiquitin-protein ligase NEDD4-like (NEDD4-L) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain E3 ubiquitin-protein ligase NEDD4-like (NEDD4-L), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of E3 ubiquitin-protein ligase NEDD4-like (NEDD4-L) in the samples is then determined by comparing the OD of the samples to the standard curve.