Human NEF3 (Neurofilament 3) ELISA Kit | ELK1883

Human NEF3 (Neurofilament 3) ELISA Kit | ELK1883 | Gentaur UK, US & Europe Distribution

Alternative Names: NEFM; NF-M; NFM; Neurofilament, Medium Polypeptide; 160 kDa neurofilament protein; Neurofilament triplet M protein

Species: Human

Assay Type: Sandwich

Sensitivity: 6.1 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: serum, plasma, tissue homogenates, cerebrospinal fluid and other biological fluids

Assay Lenght: 3.5h

Research Area: Neuro science;Developmental science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neurofilament 3 (NEF3) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Neurofilament 3 (NEF3) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neurofilament 3 (NEF3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neurofilament 3 (NEF3) in the samples is then determined by comparing the OD of the samples to the standard curve.