Human NEFA (Non-Esterified Fatty Acid) ELISA Kit | ELK8710

Human NEFA (Non-Esterified Fatty Acid) ELISA Kit | ELK8710 | Gentaur UK, US & Europe Distribution

Alternative Names: Non-Esterified Fatty Acid; Non-ester fatty acid; NEFA

Species: Human

Assay Type: Competitive Inhibition

Sensitivity: 3.55 nmol/ml

Standard: 400nmol/ml

Detection Range: 6.25-400 nmol/ml

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Assay Lenght: 2h

Research Area: Kidney diseases

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Non-Esterified Fatty Acid (NEFA) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Non-Esterified Fatty Acid (NEFA) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Non-Esterified Fatty Acid (NEFA) in the samples is then determined by comparing the OD of the samples to the standard curve.