Human NEP (Neprilysin) ELISA Kit | ELK2196

Human NEP (Neprilysin) ELISA Kit | ELK2196 | Gentaur UK, US & Europe Distribution

Alternative Names: CD10; MME; CALLA; SFE; Atriopeptidase; Enkephalinase; Skin fibroblast elastase; Common Acute Lymphoblastic Leukemia Antigen; Membrane Metallo-Endopeptidase

Species: Human

Assay Type: Sandwich

Sensitivity: 11.9 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: serum, plasma, cerebrospinal fluid and other biological fluids

Assay Lenght: 3.5h

Research Area: Enzyme & Kinase;Neuro science

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Neprilysin (NEP) . Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Neprilysin (NEP) . Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Neprilysin (NEP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Neprilysin (NEP) in the samples is then determined by comparing the OD of the samples to the standard curve.